"Version: 20210205"--Title page verso.Includes bibliographical references.1. Introduction and overview -- 1.1. Visualizing biological molecules to understand life's principles -- 1.2. Recovery of 3D structures from images of weak-phase objects2. Sample preparation -- 2.1. Overview -- 2.2. Initial screening of samples in negative stain -- 2.3. Standard method of making grids for cryo-EM -- 2.4. …